<?xml version="1.0"  ?>
<EXPERIMENT_PACKAGE_SET>
    <EXPERIMENT_PACKAGE>
        <EXPERIMENT accession="ERX3980916"
                    alias="ena-EXPERIMENT-UNIVERSITY OF HOHENHEIM-06-03-2020-13:37:12:076-1"
                    center_name="UNIVERSITY OF HOHENHEIM">
            <IDENTIFIERS>
                <PRIMARY_ID>ERX3980916</PRIMARY_ID>
            </IDENTIFIERS>
            <TITLE>Illumina MiSeq sequencing</TITLE>
            <STUDY_REF accession="ERP120343">
                <IDENTIFIERS>
                    <PRIMARY_ID>ERP120343</PRIMARY_ID>
                    <EXTERNAL_ID namespace="BioProject">PRJEB37054
                    </EXTERNAL_ID>
                </IDENTIFIERS>
            </STUDY_REF>
            <DESIGN>
                <DESIGN_DESCRIPTION/>
                <SAMPLE_DESCRIPTOR accession="ERS4372624">
                    <IDENTIFIERS>
                        <PRIMARY_ID>ERS4372624</PRIMARY_ID>
                        <EXTERNAL_ID namespace="BioSample">SAMEA6608408
                        </EXTERNAL_ID>
                    </IDENTIFIERS>
                </SAMPLE_DESCRIPTOR>
                <LIBRARY_DESCRIPTOR>
                    <LIBRARY_NAME>unspecified</LIBRARY_NAME>
                    <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
                    <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
                    <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
                    <LIBRARY_LAYOUT>
                        <SINGLE/>
                    </LIBRARY_LAYOUT>
                </LIBRARY_DESCRIPTOR>
            </DESIGN>
            <PLATFORM>
                <ILLUMINA>
                    <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
                </ILLUMINA>
            </PLATFORM>
        </EXPERIMENT>
        <SUBMISSION accession="ERA2402167"
                    alias="ena-SUBMISSION-UNIVERSITY OF HOHENHEIM-06-03-2020-13:27:09:756-1"
                    center_name="UNIVERSITY OF HOHENHEIM"
                    lab_name="European Nucleotide Archive">
            <IDENTIFIERS>
                <PRIMARY_ID>ERA2402167</PRIMARY_ID>
            </IDENTIFIERS>
            <TITLE>Submitted by UNIVERSITY OF HOHENHEIM on 06-MAR-2020</TITLE>
        </SUBMISSION>
        <Organization type="center">
            <Name abbr="University of Hohenheim">University of Hohenheim</Name>
        </Organization>
        <STUDY accession="ERP120343"
               alias="ena-STUDY-UNIVERSITY OF HOHENHEIM-04-03-2020-12:54:47:240-944"
               center_name="UNIVERSITY OF HOHENHEIM">
            <IDENTIFIERS>
                <PRIMARY_ID>ERP120343</PRIMARY_ID>
                <EXTERNAL_ID namespace="BioProject">PRJEB37054</EXTERNAL_ID>
            </IDENTIFIERS>
            <DESCRIPTOR>
                <STUDY_TITLE>The microbial load, diversity and composition of
                    the wine microbiota is affected by wine type and
                    environmental-stress factors
                </STUDY_TITLE>
                <STUDY_TYPE existing_study_type="Other"/>
                <STUDY_ABSTRACT>In order to improve the understanding of the
                    composition, organization and temporal dynamics of the wine
                    microbiota, the relative and absolute bacterial wine
                    microbiota composition during the first week of
                    fermentation was determined, including distinct red and
                    white wine cultivars, by 16S rRNA gene amplicon sequencing.
                </STUDY_ABSTRACT>
                <CENTER_PROJECT_NAME>Wine microbiota analysis during
                    fermentation
                </CENTER_PROJECT_NAME>
                <STUDY_DESCRIPTION>In order to improve the understanding of the
                    composition, organization and temporal dynamics of the wine
                    microbiota, the relative and absolute bacterial wine
                    microbiota composition during the first week of
                    fermentation was determined, including distinct red and
                    white wine cultivars, by 16S rRNA gene amplicon sequencing.
                </STUDY_DESCRIPTION>
            </DESCRIPTOR>
            <STUDY_ATTRIBUTES>
                <STUDY_ATTRIBUTE>
                    <TAG>ENA-FIRST-PUBLIC</TAG>
                    <VALUE>2020-05-31</VALUE>
                </STUDY_ATTRIBUTE>
                <STUDY_ATTRIBUTE>
                    <TAG>ENA-LAST-UPDATE</TAG>
                    <VALUE>2020-03-04</VALUE>
                </STUDY_ATTRIBUTE>
            </STUDY_ATTRIBUTES>
        </STUDY>
        <SAMPLE accession="ERS4372624" alias="BAC1.D1.0.32A"
                center_name="UNIVERSITY OF HOHENHEIM">
            <IDENTIFIERS>
                <PRIMARY_ID>ERS4372624</PRIMARY_ID>
                <EXTERNAL_ID namespace="BioSample">SAMEA6608408</EXTERNAL_ID>
            </IDENTIFIERS>
            <TITLE>Vitis vinifera</TITLE>
            <SAMPLE_NAME>
                <TAXON_ID>29760</TAXON_ID>
                <SCIENTIFIC_NAME>Vitis vinifera</SCIENTIFIC_NAME>
                <COMMON_NAME>wine grape</COMMON_NAME>
            </SAMPLE_NAME>
            <SAMPLE_ATTRIBUTES>
                <SAMPLE_ATTRIBUTE>
                    <TAG>amount or size of sample collected</TAG>
                    <VALUE>50</VALUE>
                    <UNITS>L</UNITS>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>collection date</TAG>
                    <VALUE>2015-09-28</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>collection day</TAG>
                    <VALUE>1</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>collection hours</TAG>
                    <VALUE>0</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>environment (biome)</TAG>
                    <VALUE>berry plant</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>environment (feature)</TAG>
                    <VALUE>grape plant</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>environment (material)</TAG>
                    <VALUE>wine must</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>geographic location (country and/or sea)</TAG>
                    <VALUE>Germany</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>geographic location (latitude)</TAG>
                    <VALUE>48.71 N</VALUE>
                    <UNITS>DD</UNITS>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>geographic location (longitude)</TAG>
                    <VALUE>9.12 E</VALUE>
                    <UNITS>DD</UNITS>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>investigation type</TAG>
                    <VALUE>metagenome</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>multiplex identifiers</TAG>
                    <VALUE>TAGATCGCTCGCCTTA</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>pcr primers</TAG>
                    <VALUE>GTGCCAGCMGCCGCGGTAAGGACTACHVGGGTWTCTAAT</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>plant-associated environmental package</TAG>
                    <VALUE>plant-associated</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>project name</TAG>
                    <VALUE>wine must microbiota analysis during fermentation
                    </VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>sample storage temperature</TAG>
                    <VALUE>-80</VALUE>
                    <UNITS>°C</UNITS>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>sample volume or weight for DNA extraction</TAG>
                    <VALUE>0.5</VALUE>
                    <UNITS>g</UNITS>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>sequencing method</TAG>
                    <VALUE>Illumina MiSeq</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>subspecific genetic lineage</TAG>
                    <VALUE>Bacchus1</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>target subfragment</TAG>
                    <VALUE>16S rRNA gene</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>ENA-FIRST-PUBLIC</TAG>
                    <VALUE>2020-05-31</VALUE>
                </SAMPLE_ATTRIBUTE>
                <SAMPLE_ATTRIBUTE>
                    <TAG>ENA-LAST-UPDATE</TAG>
                    <VALUE>2020-03-06</VALUE>
                </SAMPLE_ATTRIBUTE>
            </SAMPLE_ATTRIBUTES>
        </SAMPLE>
        <Pool>
            <Member member_name="" accession="ERS4372624"
                    sample_name="BAC1.D1.0.32A" sample_title="Vitis vinifera"
                    spots="39323" bases="11552099" tax_id="29760"
                    organism="Vitis vinifera">
                <IDENTIFIERS>
                    <PRIMARY_ID>ERS4372624</PRIMARY_ID>
                    <EXTERNAL_ID namespace="BioSample">SAMEA6608408
                    </EXTERNAL_ID>
                </IDENTIFIERS>
            </Member>
        </Pool>
        <RUN_SET>
            <RUN accession="FAKEID1"
                 alias="ena-RUN-UNIVERSITY OF HOHENHEIM-06-03-2020-13:37:12:076-1"
                 center_name="UNIVERSITY OF HOHENHEIM" total_spots="39323"
                 total_bases="11552099" size="3914295" load_done="true"
                 published="2020-06-01 17:54:43" is_public="true"
                 cluster_name="public" static_data_available="1">
                <IDENTIFIERS>
                    <PRIMARY_ID>FAKEID1</PRIMARY_ID>
                </IDENTIFIERS>
                <TITLE>Illumina MiSeq sequencing</TITLE>
                <EXPERIMENT_REF accession="ERX3980916">
                    <IDENTIFIERS>
                        <PRIMARY_ID>ERX3980916</PRIMARY_ID>
                    </IDENTIFIERS>
                </EXPERIMENT_REF>
                <RUN_ATTRIBUTES>
                    <RUN_ATTRIBUTE>
                        <TAG>ENA-FIRST-PUBLIC</TAG>
                        <VALUE>2020-05-31</VALUE>
                    </RUN_ATTRIBUTE>
                    <RUN_ATTRIBUTE>
                        <TAG>ENA-LAST-UPDATE</TAG>
                        <VALUE>2020-03-06</VALUE>
                    </RUN_ATTRIBUTE>
                </RUN_ATTRIBUTES>
                <Pool>
                    <Member member_name="" accession="ERS4372624"
                            sample_name="BAC1.D1.0.32A"
                            sample_title="Vitis vinifera" spots="39323"
                            bases="11552099" tax_id="29760"
                            organism="Vitis vinifera">
                        <IDENTIFIERS>
                            <PRIMARY_ID>ERS4372624</PRIMARY_ID>
                            <EXTERNAL_ID namespace="BioSample">SAMEA6608408
                            </EXTERNAL_ID>
                        </IDENTIFIERS>
                    </Member>
                </Pool>
                <SRAFiles>
                    <SRAFile cluster="public" filename="FAKEID1"
                             url="https://sra-download.ncbi.nlm.nih.gov/traces/era16/ERR/ERR3978/FAKEID1"
                             size="3915680" date="2020-06-01 19:51:45"
                             md5="d92e4c21e26e5f2bd2cdaf56cfcfeaa0"
                             semantic_name="run" supertype="Primary ETL"
                             sratoolkit="1">
                        <Alternatives
                                url="https://sra-download.ncbi.nlm.nih.gov/traces/era16/ERR/ERR3978/FAKEID1"
                                free_egress="worldwide" access_type="anonymous"
                                org="NCBI"/>
                        <Alternatives
                                url="https://sra-pub-run-odp.s3.amazonaws.com/sra/FAKEID1/FAKEID1"
                                free_egress="worldwide" access_type="anonymous"
                                org="AWS"/>
                        <Alternatives
                                url="gs://sra-pub-run-8/FAKEID1/FAKEID1.1"
                                free_egress="gs.US" access_type="gcp identity"
                                org="GCP"/>
                    </SRAFile>
                </SRAFiles>
                <CloudFiles>
                    <CloudFile filetype="run" provider="gs" location="gs.US"/>
                    <CloudFile filetype="run" provider="s3"
                               location="s3.us-east-1"/>
                </CloudFiles>
                <Statistics nreads="1" nspots="39323">
                    <Read index="0" count="39323" average="293.77"
                          stdev="20.23"/>
                </Statistics>
                <Bases cs_native="false" count="11552099">
                    <Base value="A" count="3143257"/>
                    <Base value="C" count="2405184"/>
                    <Base value="G" count="3867631"/>
                    <Base value="T" count="2136027"/>
                    <Base value="N" count="0"/>
                </Bases>
            </RUN>
        </RUN_SET>
    </EXPERIMENT_PACKAGE>
</EXPERIMENT_PACKAGE_SET>
